Single Firefly Luciferase Reporter Gene Assay Kit

Single Firefly Luciferase Reporter Gene Assay Kit

Author: ApexBio

shortsummary:

Single luciferase reporter gene test system, single enzyme reporter gene test

Con A

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In stock

halt protease and phosphatase inhibitor cocktail

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Dry ice

description:

Firefly luciferase is a protein with a molecular weight of about 61 kD that catalyzes the oxidation of the substrate luciferin to oxyluciferin in the presence of ATP, oxygen and magnesium ions. During the oxidation of Luciferin, a bioluminescence with a wavelength of about 560 nm is emitted, which can be determined by a luminometer or liquid flash analyzer. With luciferin and luciferase as bioluminescent systems, gene expression can be detected very sensitively and efficiently. Generally, the transcriptional regulatory element of the gene of interest or the 5' promoter region is cloned upstream of luciferase, or the 3'-UTR region is cloned downstream of luciferase, etc., to construct a reporter gene plasmid, transfect the cells and treat the cells with appropriate drugs, and finally determine the luciferase activity of the cell lysate and judge the transcriptional regulation effect of drug treatment on the target gene by the level of luciferase activity (Fig 1).

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APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 18817 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.